Authors
Abstract
Stress environment of space flights including hypergravity and microgravity effect cell functions and processes. We studied effect of hypergravity and flight stress on function, death, growth and cellular damage of five cell lines including human vascular endothelial cells, rat bone marrow stromal cells, mouse embryonic fibroblast cells, PC12 cells and pancreas islands in Biological payload of 3th sounding rocket by NO, LDH, MTT and insulin assay kits. Our results indicated that effect of hypergravity increase significantly cellular death and NO secretion but we found no changes in insulin secretion and pancreas function.
Keywords
[1] Clement, G. and Slenzka, K., Fundamentals of Space Biology Research on Cells, Animals, and Plant in Space, Springer, 2006.
[2] Takaoki, M., et al., “Development and Operation of Cultured Cell Experiment with TR-IA Sounding Rocket,” Biological Science Space, Vol. 12, No. 3, 1998, pp. 320-321.
[3] Gurkin, L. W., “The NASA Sounding Rocket Program and Space Sciences,” ASGSB Bulletin, Vol. 6, No. 1, 1992, pp. 113-120.
[4] Jongkind, J. F., Visser, P. and Verkerk, A., Somatic Cell Fusion During Sounding Rocket Flight. Life Sciences Experiments Performed on Sounding Rockets (1985-1994): European Space Agency, ESA SP-1206, ISBN: 92-9092-423-3, 1997, p. 99.
[5] Freshney, R. I., Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, Wilely, 2005.
[6] Hansen, W. A. and et al., “Supravital Dithizone Staining in the Isolation of Human and Rat Pancreatic Islets,” Diabetes Res., Vol. 10, No. 2, 1989, pp.53-7.
[7] Mirand, K. M., Espey, M. G. and Wink, D. A., “A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite,” Nitric Oxide, Vol. 5, No.1, 2001, pp. 62-71.
[8] Legrand, C., et. Al., “Lactate Dehydrogenase (LDH) Activity of the Number of Dead Cells in the Medium of Cultured Eukaryotic Cells as Marker,” Journal of Biotechnology, Vol. 25, No. 3, 1992, pp. 231–243.
[9] Matsuoka, M., “Cell Proliferation and Cytotoxicity Assay. Biochem,” Pharmacol, Vol. 59, 2000, p. 1573.
[10] Space Studies Board, National Reaserch Council, A Strategy for Research in Space Biology and Medicine in the New Century, National Academy Press, Washington, D. C., 1998.
[11] Sun, J., Zhang, X., Broderick, M. and Fein, H., “Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay,” Sensors, Vol. 3, No. 8, 2003, pp. 276-84.
[13] Blaber, A. P., Goswami, N., Bondar, R. L., Kassam, M. S., “Impairment of Cerebral Blood Flow Regulation in Astronauts with Orthostatic Intolerance after Flight,” Stroke. Vol. 42, No. 7, 2011, pp. 1844-50.
[14] Strollo, F., “Hormonal Changes in Humans During Spaceflight,” Advances Space Biology and Medicine, Vol.7, 1999, pp. 99-129.
)