بررسی تغییرات بیان ژن RKIP در رده سلولی MDA-MB-231 سرطان سینه پس از تیمار بی‌وزنی

نویسندگان

پژوهشگاه هوافضا

چکیده

جاذبه از مهمترین نیروهای وارده بر موجودات زنده روی زمین است که در طول حیات، بر سلول­ها، مولکول­ها و در نتیجه بر کل ارگانیسم تاثیرگذار است. هدف از این مطالعه، بررسی اثر بی وزنی شبیه سازی شده بر تغییرات بیان ژن سرکوب گر متاستاز RKIP در رده سلولی MDA-MB-231 سرطان سینه می­باشد.
مواد و روش ­ها: به منظور اعمال بی وزنی، سلول­های سرطانی، به مدت یک روز و سه روز بر روی دستگاه کلینواستت قرار گرفتند. سپس RNA از سلول­ ها استخراج و تغییرات بیان ژن RKIP توسط qReal time PCR سنجش گردید.
نتایج: یافته ­ها نشان دادند که بی­وزنی به مدت یک روز، سطح بیان ژن RKIP را کاهش داده اما با ادامه اعمال بی وزنی تا سه روز، بیان به سطح کنترل بازگشته است.
نتیجه گیری: با بهینه سازی مدت زمان تیمار بی وزنی احتمالاً شاهد تغییرات قابل توجه در بیان ژن RKIP بوده تا از این طریق بتوان گام­هایی را در کشف مکانیسم ­های درگیر در سرطان و درمان آن برداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of RKIP gene expression changes of MDA-MB-231 breast cancer cell line after microgravity treatment

نویسندگان [English]

  • Maryam Salavatifar
  • زهرا حاج‌ابراهیمی
ARI

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