cellular-molecular biology
Zahra Hajebrahimi
Articles in Press, Accepted Manuscript, Available Online from 30 December 2023
Abstract
Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast ...
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Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast cancer cell line was studied under simulated microgravity conditions using Clinostat for one and three days using real-time PCR technique and GAPDH as reference gene . Also, the effect of microgravity on cells was evaluated by the MTT test. MTT data showed that microgravity for one and three days has no significant effects on cell proliferation and metabolic activity. Also, microgravity for one day led to a doubling of E-cadherin gene expression. The continuation of microgravity for three days led to a further increase. As a result, microgravity can lead to the reduction of the cancerous phenotype in breast cancer. Therefore, studying cancer cells in a microgravity environment can help us understand the mechanisms that lead to cancer and its progression. Also, these studies can lead to the development of new treatment methods for cancer treatment.
physiology and space medicine (astrobiology)
Vajihe Zarrinpour; Zahrs Hajebrahimi
Volume 15, Issue 4 , December 2022, , Pages 89-96
Abstract
A study of the effect of microgravity on the endothelial progenitor cells is useful both in understanding cardiac changes in astronauts and in using microgravity as angiogenic stimuli. The aim of the present study was to investigate the effect of microgravity on VEGFR-2 and CD34 angiogenesis markers. ...
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A study of the effect of microgravity on the endothelial progenitor cells is useful both in understanding cardiac changes in astronauts and in using microgravity as angiogenic stimuli. The aim of the present study was to investigate the effect of microgravity on VEGFR-2 and CD34 angiogenesis markers. Following extraction of progenitor cells from peripheral blood and its confirmation, gene expression was assessed by real-time PCR, and cell viability was assessed by MTT assay. The extracted cells were endothelial progenitor cells in terms of shape and surface markers CD31 and CD144. Microgravity increased the VEGFR-2 gene expression by 3.5 times after 24 hours. CD34 expression increased by 50% after 3 h but reached control level after 24 hours. Microgravity appears to have a positive effect on the expression of angiogenic markers and stimulation of endothelial progenitor cells, and it may be used as a new environment to differentiate these cells into blood vessels and to treat heart disease.
physiology and space medicine (astrobiology)
Zahra Hajebrahimi; Maryam Salavatifar
Volume 14, Issue 4 , December 2021, , Pages 61-66
Abstract
Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study ...
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Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study was to investigate the gene expression of CD44 in MDA-MB-231cell line of breast cancer in microgravity conditioncell line was proliferated under normal gravity and microgravity (1 and 3 days) using 2-D clinostat. Gene expression was measured using real-time PCR technique. SMG increased gene expression (100%) after 1 day and decreased it (15%) during 3 days in comparison to the control samples. It seems that the response of cancer cells to microgravity is time dependent and simulated microgravity treatment for 3 days may have a positive effect on cancer characteristics of MDA-MB-231 cell line in order to decrease the expression of CD44.
physiology and space medicine (astrobiology)
maryam salavatifar; Zahra Hajebrahimi
Volume 12, Issue 1 , April 2019, , Pages 69-76
Abstract
Introduction: Gravity is one of the most important forces exposed to the organisms on the earth which affects the cells, molecules and thus the entire of organism. The purpose of this study is to investigate the effect of the simulated microgravity on the changes of RKIP metastasis suppressor gene expression ...
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Introduction: Gravity is one of the most important forces exposed to the organisms on the earth which affects the cells, molecules and thus the entire of organism. The purpose of this study is to investigate the effect of the simulated microgravity on the changes of RKIP metastasis suppressor gene expression on MDA-MB-231 breast cancer cell line.Materials and Methods: In order to impose microgravity state, cancer cells were placed on clinostat for one and three days. Then RNA was extracted from the cells and RKIP gene expression changes were evaluated by qReal time PCR.Results: The findings showed that the microgravity during one day reduced the RKIP expression level, but with continuing up to three days, the expression returned to the control level.Conclusion: By optimizing the duration of microgravity, it can be likely observed the significant effects on the RKIP gene expression changes and so that useful steps can be taken to discover the cancer mechanisms and its treatment.
Vahid Nikbakht; Ali Kazemi; Zahra Hajebrahimi; Neda Khaledi; Mohammad Asadi golzar
Volume 9, Issue 4 , April 2017, , Pages 63-68
Abstract
Endothelial cells differently respond to microgravity condition. The evolution of these responses leads to a more clear understanding of cardiovascular diseases in both gravity and micro-gravity conditions. The aim of this study was to investigate the effects of microgravity condition on serum levels ...
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Endothelial cells differently respond to microgravity condition. The evolution of these responses leads to a more clear understanding of cardiovascular diseases in both gravity and micro-gravity conditions. The aim of this study was to investigate the effects of microgravity condition on serum levels of vascular endothelial growth factor as a motivator for angiogenesis and vasculogenesis processes. A total of 20 rats were randomly divided into two groups including control group and suspension group. The results showed that the VEGF levels of suspension group were higher than control group but it was not significant (P> 0.05). It seems that the hindlimb suspension model used for stimulating microgravity condition does not affect the VEGF in serum levels in male Wistar rats. This may be the result of the body's adaptive response after six weeks. Another probable reason is that VEGF does not play an effective role in cardiovascular changes in microgravity condition. Also the influences of other factor may affect the results.
Z. Hajebrahimi; L. Alidoust; M. Arabian; E. Alavi; M. Ebrahimi; M. Bahrami
Volume 6, Issue 4 , January 2014, , Pages 37-42
Abstract
Stress environment of space flights including hypergravity and microgravity effect cell functions and processes. We studied effect of hypergravity and flight stress on function, death, growth and cellular damage of five cell lines including human vascular endothelial cells, rat bone marrow stromal cells, ...
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Stress environment of space flights including hypergravity and microgravity effect cell functions and processes. We studied effect of hypergravity and flight stress on function, death, growth and cellular damage of five cell lines including human vascular endothelial cells, rat bone marrow stromal cells, mouse embryonic fibroblast cells, PC12 cells and pancreas islands in Biological payload of 3th sounding rocket by NO, LDH, MTT and insulin assay kits. Our results indicated that effect of hypergravity increase significantly cellular death and NO secretion but we found no changes in insulin secretion and pancreas function.
