physiology and space medicine (astrobiology)
maryam salavatifar
Volume 17, Issue 1 , March 2024, , Pages 63-71
Abstract
Earth's gravity is one of the forces that constantly affects living organisms. It's changes can affect organisms behavior and performance. Study of such effects can lead to the discovery of new practical methods. Heavy metal toxicity have a lot of risks to human health. They sometimes act as a pseudo ...
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Earth's gravity is one of the forces that constantly affects living organisms. It's changes can affect organisms behavior and performance. Study of such effects can lead to the discovery of new practical methods. Heavy metal toxicity have a lot of risks to human health. They sometimes act as a pseudo element of the body and they may even interfere with metabolic processes. Cadmium as one of the most hazardous heavy metals, cause defects in DNA repair following the induction of oxidative stress, DNA damage and may lead to cancer. Several methods have been introduced to remove heavy metals from drinking water. Bioremoval by probiotic is one of these safe methods. In this study Lactobacillus acidophilus effect on cadmium bioremoval was measured in microgravity and Mars gravity conditions. The results demonstrated that the 24-hour water treatment by L. acidophilus removed 43.77% of the cadmium concentration in the Earth gravity, 54.74% under microgravity and 54.84% in Mars gravity. Statistical analysis showed that L. acidophilus was effective in cadmium bioremoval and this ability was maintained even with gravity changes. Therefore, this bacterium can be used to remove heavy metal pollution during space missions in order to maintain the health of astronauts.
physiology and space medicine (astrobiology)
Maryam Salavatifar; Nilofar Mosallaei; Ali Hatef Salmanian
Volume 15, Issue 4 , December 2022, , Pages 97-106
Abstract
Gravity is an influential force on living organisms on earth, including microorganisms. E. coli O157: H7 causes complications such as bloody diarrhea and hemolytic uremic syndrome in humans and immunization is the main way to fight it. Subunit B of Shiga-like toxin type 2 (STX2B), binding agent of bacterial ...
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Gravity is an influential force on living organisms on earth, including microorganisms. E. coli O157: H7 causes complications such as bloody diarrhea and hemolytic uremic syndrome in humans and immunization is the main way to fight it. Subunit B of Shiga-like toxin type 2 (STX2B), binding agent of bacterial toxin to target cells, is a candidate for the recombinant vaccine to prevent disease. Due to the quantitative and qualitative increase in the expression of some recombinant proteins under microgravity, the STX2B recombinant protein expression was examined under simulated microgravity conditions on clinostat device. After confirmation of the recombinant by immunological methods, its expression was performed under microgravity and after the protein purification by chromatographic column, its amount was measured. The results showed that despite the decrease in expression under microgravity, which was probably due to lack of proper aeration of the culture medium, recombinant protein was still produced under microgravity condition.
physiology and space medicine (astrobiology)
Zahra Hajebrahimi; Maryam Salavatifar
Volume 14, Issue 4 , December 2021, , Pages 61-66
Abstract
Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study ...
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Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study was to investigate the gene expression of CD44 in MDA-MB-231cell line of breast cancer in microgravity conditioncell line was proliferated under normal gravity and microgravity (1 and 3 days) using 2-D clinostat. Gene expression was measured using real-time PCR technique. SMG increased gene expression (100%) after 1 day and decreased it (15%) during 3 days in comparison to the control samples. It seems that the response of cancer cells to microgravity is time dependent and simulated microgravity treatment for 3 days may have a positive effect on cancer characteristics of MDA-MB-231 cell line in order to decrease the expression of CD44.
physiology and space medicine (astrobiology)
maryam salavatifar; Zahra Hajebrahimi
Volume 12, Issue 1 , April 2019, , Pages 69-76
Abstract
Introduction: Gravity is one of the most important forces exposed to the organisms on the earth which affects the cells, molecules and thus the entire of organism. The purpose of this study is to investigate the effect of the simulated microgravity on the changes of RKIP metastasis suppressor gene expression ...
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Introduction: Gravity is one of the most important forces exposed to the organisms on the earth which affects the cells, molecules and thus the entire of organism. The purpose of this study is to investigate the effect of the simulated microgravity on the changes of RKIP metastasis suppressor gene expression on MDA-MB-231 breast cancer cell line.Materials and Methods: In order to impose microgravity state, cancer cells were placed on clinostat for one and three days. Then RNA was extracted from the cells and RKIP gene expression changes were evaluated by qReal time PCR.Results: The findings showed that the microgravity during one day reduced the RKIP expression level, but with continuing up to three days, the expression returned to the control level.Conclusion: By optimizing the duration of microgravity, it can be likely observed the significant effects on the RKIP gene expression changes and so that useful steps can be taken to discover the cancer mechanisms and its treatment.
