physiology and space medicine (astrobiology)
Vajihe Zarrinpour; Zahrs Hajebrahimi
Volume 15, Issue 4 , December 2022, , Pages 89-96
Abstract
A study of the effect of microgravity on the endothelial progenitor cells is useful both in understanding cardiac changes in astronauts and in using microgravity as angiogenic stimuli. The aim of the present study was to investigate the effect of microgravity on VEGFR-2 and CD34 angiogenesis markers. ...
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A study of the effect of microgravity on the endothelial progenitor cells is useful both in understanding cardiac changes in astronauts and in using microgravity as angiogenic stimuli. The aim of the present study was to investigate the effect of microgravity on VEGFR-2 and CD34 angiogenesis markers. Following extraction of progenitor cells from peripheral blood and its confirmation, gene expression was assessed by real-time PCR, and cell viability was assessed by MTT assay. The extracted cells were endothelial progenitor cells in terms of shape and surface markers CD31 and CD144. Microgravity increased the VEGFR-2 gene expression by 3.5 times after 24 hours. CD34 expression increased by 50% after 3 h but reached control level after 24 hours. Microgravity appears to have a positive effect on the expression of angiogenic markers and stimulation of endothelial progenitor cells, and it may be used as a new environment to differentiate these cells into blood vessels and to treat heart disease.
physiology and space medicine (astrobiology)
Ehsan Siami; Reza Mohammadi; Vajiheh Zarrinpour
Volume 15, Issue 1 , March 2022, , Pages 41-49
Abstract
This study evaluates the expression level of genes that change by pressure changes in astronautics and diving disorders such as decompression sickness. This study was performed on five skilled divers to analyze the expression level of TLR-2, HSP-72, IL-1b, NF-kB, and MPO genes that are responsible for ...
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This study evaluates the expression level of genes that change by pressure changes in astronautics and diving disorders such as decompression sickness. This study was performed on five skilled divers to analyze the expression level of TLR-2, HSP-72, IL-1b, NF-kB, and MPO genes that are responsible for pro-inflammatory conditions. In addition, the expression level of DRD4 and BNIP3 genes (Astronaut index genes that were previously measured in a spacecraft study on mice) was analyzed on native Iranian samples. Blood samples were taken half an hour after diving, RNA was extracted, and cDNA was synthesized. Then, real-time PCR was done. Based on the results obtained using the T-Student statistical method, it was found that the expression level of all genes except MPO and TLR-2 genes increased significantly after exposure to pressure changes and diving activities. These conditions can be a permanent effect after pressure changes and can be considered an indicator for healthy people under stress in diving and astronautics.