نوع مقاله : مقالة تحقیقی (پژوهشی)
نویسندگان
1 دانشیار، گروه پژوهشی فیزیولوژی هوافضایی، پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران
2 استادیار گروه پژوهشی فیزیولوژی هوافضایی، پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران
چکیده
بی وزنی بر رشد و متاستاز سلول‌های توموری اثر می گذارد. با این وجود اساس مولکولی آن شناخته نشده است. به دلیل بیان بالای مولکول CD44 در تومورهای بازال تهاجمی سرطان سینه، در سال های اخیر موضوع بسیاری از مطالعات بوده است. هدف از مطالعه حاضر بررسی تغییرات بیانی ژن CD44 در رده سلولی MDA-MB-231 در شرایط بی‌وزنی بود. رده سلولی در شرایط جاذبه طبیعی و بی‌وزنی 1 و 3 روز توسط دستگاه کلینواستت دو بعدی تکثیر شد. بیان ژن با Real-time PCR اندازه‌گیری شد. میزان بیان ژن پس از یک روز به میزان 100٪ افزایش و پس از 3 روز به میزان 15٪ کاهش می یابد. به نظر می‌رسد که پاسخ سلول‌های سرطانی به بی‌وزنی وابسته به زمان باشد و اعمال آن برای مدت سه روز ممکن است اثرات مثبتی بر کاهش فنوتیپ سرطانی در این رده سلولی از لحاظ کاهش بیان مولکول CD44 داشته باشد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
CD44 expression changes in MDA-MB-231 cell line of breast cancer after exposure to 2-D clinorotation
نویسندگان [English]
- Zahra Hajebrahimi 1
- Maryam Salavatifar 2
1 Associate Professor, Aerospace Physiology Research Group, Aerospace Research Institute, Ministry of Science, Research and Technology, Tehran, Iran
2 assistant professor, Aerospace Physiology Research Group, Aerospace Research Institute, Ministry of Science, Research and Technology, Tehran, Iran
چکیده [English]
Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study was to investigate the gene expression of CD44 in MDA-MB-231cell line of breast cancer in microgravity conditioncell line was proliferated under normal gravity and microgravity (1 and 3 days) using 2-D clinostat. Gene expression was measured using real-time PCR technique. SMG increased gene expression (100%) after 1 day and decreased it (15%) during 3 days in comparison to the control samples. It seems that the response of cancer cells to microgravity is time dependent and simulated microgravity treatment for 3 days may have a positive effect on cancer characteristics of MDA-MB-231 cell line in order to decrease the expression of CD44.
کلیدواژه‌ها [English]
- Breast cancer
- microgravity
- CD44
- MDA-MB-231 cell line
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