cellular-molecular biology
Zahra Hajebrahimi
Articles in Press, Accepted Manuscript, Available Online from 30 December 2023
Abstract
Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast ...
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Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast cancer cell line was studied under simulated microgravity conditions using Clinostat for one and three days using real-time PCR technique and GAPDH as reference gene . Also, the effect of microgravity on cells was evaluated by the MTT test. MTT data showed that microgravity for one and three days has no significant effects on cell proliferation and metabolic activity. Also, microgravity for one day led to a doubling of E-cadherin gene expression. The continuation of microgravity for three days led to a further increase. As a result, microgravity can lead to the reduction of the cancerous phenotype in breast cancer. Therefore, studying cancer cells in a microgravity environment can help us understand the mechanisms that lead to cancer and its progression. Also, these studies can lead to the development of new treatment methods for cancer treatment.
physiology and space medicine (astrobiology)
Zahra Hajebrahimi; Maryam Salavatifar
Volume 14, Issue 4 , December 2021, , Pages 61-66
Abstract
Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study ...
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Studies have shown that simulated microgravity (SMG) affects tumor cell growth and metastasis. However, the underlying molecular basis is still not known. In recent years, due to the high expression of CD44 in invasive basal breast tumors, it has been the subject of many studies. The aim of present study was to investigate the gene expression of CD44 in MDA-MB-231cell line of breast cancer in microgravity conditioncell line was proliferated under normal gravity and microgravity (1 and 3 days) using 2-D clinostat. Gene expression was measured using real-time PCR technique. SMG increased gene expression (100%) after 1 day and decreased it (15%) during 3 days in comparison to the control samples. It seems that the response of cancer cells to microgravity is time dependent and simulated microgravity treatment for 3 days may have a positive effect on cancer characteristics of MDA-MB-231 cell line in order to decrease the expression of CD44.
