cellular-molecular biology
Zahra Hajebrahimi
Articles in Press, Accepted Manuscript, Available Online from 30 December 2023
Abstract
Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast ...
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Simulated microgravity can change tumor cell adhesion, proliferation, invasion, and metastasis. E-cadherin molecule is a cell-to-cell adhesion regulatory molecule whose expression is reduced in most types of cancer cells. In the present study, the expression of the E-cadherin gene in the MCF-7 breast cancer cell line was studied under simulated microgravity conditions using Clinostat for one and three days using real-time PCR technique and GAPDH as reference gene . Also, the effect of microgravity on cells was evaluated by the MTT test. MTT data showed that microgravity for one and three days has no significant effects on cell proliferation and metabolic activity. Also, microgravity for one day led to a doubling of E-cadherin gene expression. The continuation of microgravity for three days led to a further increase. As a result, microgravity can lead to the reduction of the cancerous phenotype in breast cancer. Therefore, studying cancer cells in a microgravity environment can help us understand the mechanisms that lead to cancer and its progression. Also, these studies can lead to the development of new treatment methods for cancer treatment.
hossein ghadampour-vahed; Ali Kazemi; bagher minaei; zabih allah ghadampour-vahed
Volume 12, Issue 2 , September 2019, , Pages 71-78
Abstract
The purpose of this study was to investigate the effect of six weeks of HIIT under simulated microgravity condition on morphological changes of radial nerve myelin sheath in male rats. In present study, 24 male wistar rats were randomly selected and divided into four groups including: TS(training during ...
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The purpose of this study was to investigate the effect of six weeks of HIIT under simulated microgravity condition on morphological changes of radial nerve myelin sheath in male rats. In present study, 24 male wistar rats were randomly selected and divided into four groups including: TS(training during suspension:n=6), CS(suspension:n=6), T(training:n=6), C(control:n=6). The training groups run on treadmill for six weeks (five days in week). 24 hours after the last training session, the animals were prepared for tissue samples. At the end of the experiment, changes in myelin sheath were measured by Luxol fast blue. Our results showed that the percentage of changes in myelin sheath in HIIT group under simulate microgravity (p≤0.001) was highest than other groups, significantly (30/27+5/27). Based on the results, HIIT under simulated microgravity condition affected on changes in myelin sheath, Therefore, it seems that can play an effective role in improvement of the clinical status of neurological patients and decrease side effects in Astronauts.
